Skip to main content

Lance Seefeldt

Profile Picture

Biochemistry

Professor


Educational Background

PhD, Biochemistry, University of California, 1989
BS, Chemistry, University of Redlands, 1983

Biography

Trained in chemistry and biochemistry at the University of Redlands, the University of California at Riverside, and the University of Georgia. On the faculty in the Chemistry and Biochemistry Department at Utah State University since 1993.


Teaching Interests

General chemistry, general biochemistry, bioenergetics, metallobiochemistry, nitrogen fixation.

Research Interests

Biofuels, bioenergy, nitrogen fixation, food production, metals in biology.

Awards

Co-Chair, N Cycle Workshop, 2015

National Science Foundation

Council Member, Council on Chemical Sciences, Geosciences, and Biosciences, 2015

Department of Energy

Laboratory Fellow, 2013

Pacific Northwest National Laboratory

Fellow, American Association for the Advancement of Science, 2013

American Association for the Advancement of Science

Outstanding Lecturer Award, 2012

Pacific Northwest National Laboratory

D. Wynne Thorne Career Research Award, 2012

Utah State University

Science Collaborator of the Year, 2010

Energy Dynamics Laboratory, USU

USU College of Science Teacher of the Year, 2007

USU College of Science

USU College of Science Researcher of the Year, 2004

USU College of Science

Eli Lily Travel Award Recipient, 1992

The Protein Society

Outstanding Teaching Assistant of the Year in Biochemistry, 1987

University of California, Riverside

Publications - Abstracts

    Publications - Books & Book Chapters

      * Has not been peer reviewed

      Publications - Fact Sheets

        * Has not been peer reviewed

        Publications - Curriculum

          * Has not been peer reviewed

          Publications - Journal Articles

            Academic Journal

          • Davydov, R., Khadka, N., Yang, Z., Fielding, A.J, Lukoyanov, D., Dean, D.R, Seefeldt, L., Hoffman, B.M, (2016). Exploring electron/proton transfer and conformational changes in the nitrogenase MoFe protein and FeMo-cofactor through cryoreduction/EPR measurements. Israel Journal of Chemistry, 56, 841–851.
          • Paengnakorn, P., Ash, P.A, Shaw, S., Danyal, K., Chen, T., Dean, D.R, Seefeldt, L., Vincent, K.A, (2016). Infrared spectroscopy of the nitrogenase MoFe protein under electrochemical control: potential-triggered CO binding. Chem. Sci., (in press)
          • Danyal, K., Shaw, S., Page, T.R, Duval, S., Horitani, M., Marts, A.R, Lukoyanov, D., Dean, D.R, Raugei, S., Hoffman, B.M, Seefeldt, L., Antony, E., (2016). Negative cooperativity in the nitrogenase Fe protein electron delivery cycle. Proceedings of the National Academy of Sciences, 113, E5783–E5791.
          • Khadka, N., Dean, D.R, Smith, D., Hoffman, B.M, Raugei, S., Seefeldt, L., (2016). CO<sub>2</sub> reduction catalyzed by nitrogenase: pathways to formate, carbon monoxide, and methane. Inorganic Chemistry, 55:17, 8321–8330.
          • Fixen, K.R, Zheng, Y., Harris, D.F, Shaw, S., Yang, Z., Dean, D.R, Seefeldt, L., Harwood, C.S, (2016). Light-driven carbon dioxide reduction to methane by nitrogenase in a photosynthetic bacterium. Proceedings of the National Academy of Sciences, 113:36, 10163–10167.
          • Milton, R.D, Abdellaoui, S., Khadka, N., Dean, D.R, Leech, D., Seefeldt, L., Minteer, S.D, (2016). Nitrogenase bioelectrocatalysis: heterogeneous ammonia and hydrogen production by MoFe protein. Energy & Environmental Science, 9:8, 2550–2554.
          • Lukoyanov, D., Khadka, N., Yang, Z., Dean, D.R, Seefeldt, L., Hoffman, B.M, (2016). Reductive elimination of H<sub>2</sub> activates nitrogenase to reduce the N≡N triple bond: characterization of the E4(4H) Janus intermediate in wild-type enzyme. Journal of the American Chemical Society, 138:33, 10674–10683.
          • Yang, Z., Ledbetter, R., Shaw, S., Pence, N., Tokmina-Lukaszewska, M., Eilers, B., Guo, Q., Pokhrel, N., Cash, V.L, Dean, D.R, Antony, E., Bothner, B., Peters, J.W, Seefeldt, L., (2016). Evidence that the Pi release event is the rate-limiting step in the nitrogenase catalytic cycle. Biochemistry, 55, 3625–3635.
          • Brown, K.A, Harris, D.F, Wilker, M.B, Rasmussen, A., Khadka, N., Hamby, H., Keable, S., Dukovic, G., Peters, J.W, Seefeldt, L., King, P.W, (2016). Light-driven dinitrogen reduction catalyzed by a CdS:nitrogenase MoFe protein biohybrid. Science, 352:6284, 448–450.
          • Lukoyanov, D., Khadka, N., Yang, Z., Dean, D.R, Seefeldt, L., Hoffman, B.M, (2016). Reversible photoinduced reductive elimination of H<sub>2</sub> from the nitrogenase dihydride state, the E4(4H) Janus intermediate. Journal of the American Chemical Society, 138:4, 1320–1327.
          • Lukoyanov, D., Yang, Z., Khadka, N., Dean, D.R, Seefeldt, L., Hoffman, B.M, (2015). Identification of a Key Catalytic Intermediate Demonstrates That Nitrogenase Is Activated by the Reversible Exchange of N2 for H2. Journal of the American Chemical Society
          • Shaw, S., Lukoyanov, D., Danyal, K., Dean, D.R, Hoffman, B.M, Seefeldt, L., (2014). Nitrite and Hydroxylamine as Nitrogenase Substrates: Mechanistic Implications for the Pathway of N2 Reduction. Journal of the American Chemical Society, 136:36, 12776–12783.
          • Hoffman, B.M, Lukoyanov, D., Yang, Z., Dean, D.R, Seefeldt, L., (2014). Mechanism of nitrogen fixation by nitrogenase: the next stage. Chemical Reviews, 114:8, 4041–4062.
          • Smith, D., Danyal, K., Raugei, S., Seefeldt, L., (2014). Substrate channel in nitrogenase revealed by a molecular dynamics approach. Biochemistry, 53:14, 2278–2285.
          • Lukoyanov, D., Yang, Z., Duval, S., Danyal, K., Dean, D.R, Seefeldt, L., Hoffman, B.M, (2014). A confirmation of the quench-cryoannealing relaxation protocol for identifying reduction states of freeze-trapped nitrogenase intermediates. Inorganic Chemistry, 53, 3688–3693.
          • Adams, C., Godfrey, V., Wahlen, B., Seefeldt, L., Bugbee, B.G, (2013). Understanding precision nitrogen stress to optimize the growth and lipid content tradeoff in oleaginous green microalgae. Bioresource technology, 131, 188–194.
          • Wahlen, B.D, Morgan, M.R, McCurdy, A.T, Willis, R.M, Morgan, M.D, Dye, D.J, Bugbee, B.G, Wood, B.D, Seefeldt, L., (2012). Biodiesel from microalgae, yeast, and bacteria: engine performance and exhaust emissions. Energy & Fuels, 27:1, 220–228.
          • Wahlen, B.D, Willis, R.M, Seefeldt, L., (2011). Biodiesel production by simultaneous extraction and conversion of total lipids from microalgae, cyanobacteria, and wild-mixed cultures. Bioresource Technology, 102, 2724-2730.
          • Yang, Z.Y, Seefeldt, L., Dean, D.R, Cramer, S.P, George, S.J, (2011). Steric control of the Hi-CO MoFe nitrogenase complex revealed by stopped-flow infra-red spectroscopy. Angewandte Chemie International Edition, 50, 272-275.
          • Danyal, K., Mayweather, D., Dean, D.R, Seefeldt, L., Hoffman, B.M, (2010). Conformational gating of electron transfer from nitrogenase Fe protein to MoFe protein. Journal of the American Chemical Society, 132, 6894-6895.
          • Sarma, R., Barney, B.M, Keable, S., Dean, D.R, Seefeldt, L., Peters, J.W, (2010). Insights into substrate binding at FeMo-cofactor in nitrogenase from the structure of an alpha70Ile MoFe protein variant. Journal of Inorganic Biochemistry, 104, 385-389.
          • Lukoyanov, D., Yang, Z.Y, Dean, D.R, Seefeldt, L., Hoffman, B.M, (2010). Is Mo involved in hydride binding by the four-electron reduced (E4) intermediate of the nitrogenase MoFe protein? . Journal of the American Chemical Society, 132, 2526-2527.
          • Danyal, K., Inglet, B.S, Vincent, K.A, Barney, B.M, Hoffman, B.M, Armstrong, F.A, Dean, D.R, Seefeldt, L., (2010). Uncoupling nitrogenase: catalytic reduction of hydrazine to ammonia by a MoFe protein in the absence of Fe protein-ATP. Journal of the American Chemical Society, 132, 13197-13199.
          • Barney, B.M, Yurth, M.G, Dos Santos, P.C, Dean, D.R, Seefeldt, L., (2009). A substrate channel in the nitrogenase MoFe protein. Journal of Biological Inorganic Chemistry, 14, 1015-1022.
          • Harris, D.G, Seefeldt, L., (2009). An Updated Kinetic Study of the Enzyme Lactase for the Biochemistry Laboratory. Journal of Chemical Education, 86, 271.
          • Hoffman, B.M, Dean, D.R, Seefeldt, L., (2009). Climbing nitrogenase: towards a mechanism of enzymatic nitrogen fixation. Accounts of Chemical Research, 42, 609-619.
          • Seefeldt, L., Hoffman, B.M, Dean, D.R, (2009). Mechanism of Mo-dependent nitrogenase. Annual Review of Biochemistry, 78, 101-722.
          • Wahlen, B.D, Oswald, W.S, Seefeldt, L., Barney, B.M, (2009). Purification, characterization, and potential bacterial wax ester production role of an NADPH-dependent fatty aldehyde reductase from Marinobacter aquaeolei VT8 . Applied and Environmental Microbiology, 75, 2758-2764.
          • Barney, B.M, Lukoyanov, D., Igarashi, R.Y, Laryukhin, M., Yang, T.C, Dean, D.R, Hoffman, B.M, Seefeldt, L., (2009). Trapping an intermediate of dinitrogen (N2) reduction on nitrogenase. Biochemistry, 48, 9094-9102.
          • Sarma, R., Barney, B.M, Hamilton, T.L, Jones, A., Seefeldt, L., Peters, J.W, (2008). Crystal structure of the L protein of Rhodobacter sphaeroides light dependent protochlorophyllide reductase with MgADP bound: a homologue of the nitrogenase Fe protein. Biochemistry, 47, 13004-13015.
          • Wahlen, B.D, Barney, B.M, Seefeldt, L., (2008). Synthesis of biodiesel from mixed feedstocks and longer chain alcohols using an acid catalyzed method. Energy Fuels, 22, 4223-4228.
          • Seefeldt, L., (2008). Synthesis of Biodiesel from Mixed Feedstocks and Longer Chain Alcohols using an Acid Catalyzed Method. Energy and Fuels, 22, 4223.
          • Professional Journal

          • Wahlen, B.D, Morgan, M.R, McCurdy, A.T, Willis, R.M, Dye, D.J, Bugbee, B.G, Wood, B.D, Seefeldt, L., (2013). Biodiesel from microalgae, yeast, and bacteria: engine performance and exhaust emissions. Energy Fuels, 27, 220-228.
          • Duval, S., Danyal, K., Shaw, S., Lytle, A.K, Dean, D.R, Hoffman, B.M, Antony, E., Seefeldt, L., (2013). Electron transfer precedes ATP hydrolysis during nitrogenase catalysis. Proc. Natl. Acad. Sci. USA, 110, 16414-16419.
          • Appel, A.M, Seefeldt, L., (2013). Frontiers, Opportunities, and Challenges in Biochemical and Chemical Catalysis of CO2 Fixation.. Chem. Rev., 113, 6621-6658.
          • Seefeldt, L., Yang, Z.Y, Duval, S., Dean, D.R, (2013). Nitrogenase reduction of carbon-containing compounds. Biochim. Biophys. Acta, 1827, 1102-1111.
          • Hoffman, B.M, Lukoyanov, D., Dean, D.R, Seefeldt, L., (2013). Nitrogenase: a draft mechanism. Acc Chem Res, 46, 587-595.
          • Yang, Z.Y, Khadka, N., Lukoyanov, D., Hoffman, B.M, Dean, D.R, Seefeldt, L., (2013). On reversible H2 loss upon N2 binding to FeMo-cofactor of nitrogenase. Proc. Natl. Acad. Sci. USA, 110, 16327-16332.
          • Moure, V.R, Danyal, K., Wendroth, S., Muller-Santos, M., Pedrosa, F.O, Scarduelli, M., Souza, E.M, Seefeldt, L., (2013). The nitrogenase regulatory enzyme dinitrogenase reductase ADP-ribosyltransferase (DraT) is activated by direct interaction with the signal transduction protein GlnB. J. Bacteriol., 195, 279-286.
          • Adams, C., Godfrey, V., Wahlen, B., Seefeldt, L., Bugbee, B.G, (2013). Understanding precision nitrogen stress to optimize the growth and lipid content tradeoff in oleaginous green microalgae. Bioresource Technol., 131, 188-194.
          • Yang, Z.Y, Moure, V.R, Dean, D.R, Seefeldt, L., (2012). Carbon dioxide reduction to methane and coupling with acetylene to form propylene catalyzed by remodeled nitrogenase. Proc. Natl. Acad. Sci. USA, 109, 19644-19648.
          • Barney, B.M, Wahlen, B.D, Garner, E., Wei, J., Seefeldt, L., (2012). Differences in substrate specificities of five bacterial was ester synthases. Appl. Environ. Microbiol., 78, 5734-5745.
          • Seefeldt, L., Hoffman, B.M, Dean, D.R, (2012). Electron transfer in nitrogenase catalysis. Curr. Opin. Chem. Biol. , 16, 19-25.
          • George, S.J, Barney, B.M, Mitra, D., Igarashi, R.Y, Guo, Y., Dean, D.R, Cramer, S.P, Seefeldt, L., (2012). EXAFS and NRVS reveal a conformational distortion of the FeMo-cofactor in the MoFe nitrogenase propargyl alcohol complex. . J. Inorg. Biochem., 112, 85-92.
          • Mayweather, D., Danyal, K., Dean, D.R, Seefeldt, L., Hoffman, B.M, (2012). Temperature invariance of the nitrogenase electron transfer mechanism. Biochemistry, 51, 8391-8398.
          • Lukoyanov, D., Yang, Z.Y, Barney, B.M, Dean, D.R, Seefeldt, L., Hoffman, B.M, (2012). Unification of reaction pathway and kinetic scheme for N2 reduction catalyzed by nitrogenase. Proc. Natl. Acad. Sci. USA, 109, 5583-5587.
          • Doan, P.E, Telser, J., Barney, B.M, Igarashi, R.Y, Dean, D.R, Seefeldt, L., Hoffman, B.M, (2011). (57)Fe ENDOR spectroscopy and 'electron inventory' analysis of the nitrogenase E(4) intermediate suggest the metal-ion core of FeMo-cofactor cycles through only one redox couple. J. Am. Chem. Soc., 133, 17329-17340.
          • Danyal, K., Dean, D.R, Hoffman, B.M, Seefeldt, L., (2011). ) Electron transfer within nitrogenase: evidence for a deficit spending mechanism. Biochemistry, 50, 9255-9263.
          • Willis, R.M, Wahlen, B.D, Seefeldt, L., Barney, B.M, (2011). Characterization of a fatty acyl-CoA reductase from Marinobacter aquaeolei VT8: A bacterial enzyme catalyzing the reduction of fatty acyl-CoA to fatty alcohol. Biochemistry, 50, 10550-10558.
          • Lukoyanov, D., Dkianov, S.A, Yang, Z.H, Barney, B.M, Samoilova, R.I, Narasimhulu, K., Dean, D.R, Seefeldt, L., Hoffman, B.M, (2011). ENDOR/HYSCORE studies of the common intermediate trapped during nitrogenase reduction of N2H2, CH3N2H, and N2H4 support an alternating reaction pathway for N2 reduction. J. Am. Chem. Soc., 133, 11655-11664.
          • Yang, Z., Dean, D.R, Seefeldt, L., , (2011). Molybdenum nitrogenase catalyzes the reduction and coupling of CO to form hydrocarbons. J. Biol. Chem., 286, 19417-19421.

          * Has not been peer reviewed

          Publications - Literary Journal

            * Has not been peer reviewed

            Publications - MultiMedia

              * Has not been peer reviewed

              Publications - Technical Reports

              * Has not been peer reviewed

              Publications - Translations & Transcripts

                Publications - Other

                  * Has not been peer reviewed

                  Scheduled Teaching

                  CHEM 3700 - Introductory Biochemistry, Spring 2017

                  CHEM 3700 - Introductory Biochemistry, Spring 2017

                  CHEM 6760 - Principles of Bioenergetics, Spring 2017

                  CHEM 4890 - Undergraduate Biochemistry Seminar, Spring 2017

                  CHEM 3700 - Introductory Biochemistry, Summer 2016

                  CHEM 3700 - Introductory Biochemistry, Spring 2016

                  CHEM 6760 - Principles of Bioenergetics, Spring 2016

                  CHEM 4800 - Research Problems, Spring 2016

                  CHEM 3700 - Introductory Biochemistry, Summer 2015

                  CHEM 6710 - Advanced Biochemistry II, Spring 2015

                  CHEM 3700 - Introductory Biochemistry, Spring 2015

                  CHEM 4800 - Research Problems, Spring 2015

                  CHEM 4890 - Undergraduate Biochemistry Seminar, Spring 2015

                  CHEM 7770 - Special Topics in Biochemistry (Topic), Fall 2014

                  CHEM 3700 - Introductory Biochemistry, Summer 2014

                  CHEM 3700 - Introductory Biochemistry, Spring 2014

                  CHEM 6760 - Principles of Bioenergetics, Fall 2013

                  CHEM 3700 - Introductory Biochemistry, Spring 2013

                  CHEM 4890 - Undergraduate Biochemistry Seminar, Spring 2013

                  CHEM 7800 - Seminar, Fall 2012

                  CHEM 7800 - Seminar, Fall 2012

                  CHEM 4890 - Undergraduate Biochemistry Seminar, Fall 2012

                  CHEM 3700 - Introductory Biochemistry, Spring 2012

                  CHEM 6760 - Principles of Bioenergetics, Fall 2011

                  CHEM 4800 - Research Problems, Summer 2011

                  CHEM 7970 - DISSERTATION RSRCH, Spring 2011

                  CHEM 3700 - INTRO BIOCHEMISTRY, Spring 2011

                  CHEM 1220 - PRIN OF CHEMISTRY II (BPS), Spring 2011

                  CHEM 1220 - RECITATION, Spring 2011

                  CHEM 1220 - RECITATION, Spring 2011

                  CHEM 1220 - RECITATION, Spring 2011

                  CHEM 1220 - RECITATION, Spring 2011

                  CHEM 1220 - RECITATION, Spring 2011

                  CHEM 1220 - RECITATION, Spring 2011

                  CHEM 1220 - RECITATION, Spring 2011

                  CHEM 1220 - RECITATION, Spring 2011

                  CHEM 1220 - RECITATION, Spring 2011

                  CHEM 1220 - RECITATION, Spring 2011

                  CHEM 1220 - RECITATION, Spring 2011

                  CHEM 6970 - THESIS RESEARCH, Spring 2011

                  CHEM 7970 - PhD Dissertation Research, Fall 2010

                  CHEM 6970 - Thesis Research, Fall 2010


                  Graduate Students Mentored

                  Nimesh Khadka, Chemistry & Biochemistry, January 2012
                  Sudipta Shaw, Chemistry & Biochemistry, January 2012
                  Alex McCurdy, Chemistry & Biochemistry
                  Shannon Wendroth, Chemistry & Biochemistry
                  Robert Willis, Chemistry & Biochemistry
                  Zhiyong Wang, Chemistry & Biochemistry
                  Karamatulla Danayl, Chemistry & Biochemistry
                  Valerie Godfrey, Chemistry & Biochemistry 2012
                  Brad Wahlen, 2010
                  Alejandro Pabon, 2009
                  Jammi McClead, 2006
                  Robert Igarashi, 2005
                  Nathen Maeser, 2003
                  Jeannine Chan, Chemistry & Biochemistry 2001
                  Matthew Ryle, Chemistry & Biochemistry 1998
                  William Lanzilotta, Chemistry & Biochemistry 1998