A yoph ptp1b chimera shows the importance of the wpd-loop sequence to the activity, structure, and dynamics of protein tyrosine phosphatases
Gwendolyn Moise, Yalemi Morales, Victor Beaumont, Timothy Caradonna, J. Patrick Loria, Sean J. Johnson, and Alvan C. Hengge ABSTRACT: To study factors that affect WPD-loop motion in protein tyrosine phosphatases (PTPs), a chimera of PTP1B and YopH was cre...
Structure of frequency-interacting RNA helicase from neurospora crassa reveals high flexibility in a domain critical for circadian rhythm and RNA surveiillance
Morales Y., Olsen KJ, Bulcher JM, Johnson SJ ABSTRACT:The FRH (frequency-interacting RNA helicase) protein is the Neurospora crassa homolog of yeast Mtr4, an essential RNA helicase that plays a central role in RNA metabolism as an activator of the nucle
Electrocatalytic CO2 reduction catalyzed by nitrogenase MoFe and FeFe proteins
Bo Hu, Derek F. Harris, Dennis R. Dean, T. Leo Liu, Zhi-Yong Yang, Lance C. Seefeldt: Nitrogenases catalyze biological dinitrogen (N2) reduction to ammonia (NH3), and also reduce a number of non-physiological substrates, including carbon dioxide (CO2) to
MxiN differentially regulates monomeric and oligomeric species of the Shigella type three secretion system ATPase Spa47
Heather B. Case and Nicholas E. Dickenson: Shigella rely entirely on the action of a single type three secretion system (T3SS) to support cellular invasion of colonic epithelial cells and to circumvent host immune responses. The ATPase Spa47 resides at th...
Mechanism of N2 reduction catalyzed by Fe-nitrogenase involves reductive elimination of H2
Derek F. Harris, Dmitriy A. Lukoyanov, Sudipta Shaw, Phil Compton, Monika Tokmina-Lukaszewska, Brian Bothner, Neil Kelleher, Dennis R. Dean, Brian M. Hoffman, and Lance C. Seefeldt: Of the three forms of nitrogenase (Mo-nitrogenase, V-nitrogenase, and Fe-...
Phe71 in type III trypanosoomal protein rrginine methyltransferase 7 (TbPRMT7) restricts to monomethylation
Tamar B. Caceres, Abhishek Thakur, Owen M Price, Nicole Ippolito, Jun Li, Jun Qu, Orlando Acevedo and Joan Hevel ABSTRACT: Protein arginine methyltransferase 7 (PRMT7) is unique within the PRMT family as it is the only isoform known to exclusively make m
Conformational dynamics of DNA binding and Cas3 recruitment by the CRISPR RNA-guided cascade complex
Paul B. G. van Erp, Angela Patterson, Ravi Kant, Luke Berry, Sarah M. Golden, Brittney L. Forsman, Joshua Carter, Ryan N. Jackson, Brian Bothner, and Blake Wiedenheft: Bacteria and archaea rely on CRISPR (clustered regularly interspaced short palindromic
Conformational regulation of CRISPR-associated nucleases
Jackson, R., van Erp, P.B, Sternberg, S.H, Wiedenheft, B.: Adaptive immune systems in bacteria and archaea rely on small CRISPR-derived RNAs (crRNAs) to guide specialized nucleases to foreign nucleic acids. The activation of these nucleases is controlled
Thermal and elastic characterization of glassy carbon thin films by photoacoustic measurements
D.D. Markushev, J. Ordonez-Miranda, M.D. Rabasovi´c, M. Chirtoc, D.M. Todorovi´c, S.E. Bialkowski, D. Korte, and M. Franko: A portable photoacoustic device is designed and applied to measure thermal diffusivity and linear thermal expansion coefficient of
Faculty member in the College of Science, Department of Chemistry and Biochemistry